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Updated: May 19, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Sensitive localized surface plasmon resonance multiplexing protocols.

Kun Jia1, Jean L Bijeon, Pierre M Adam

  • 1Laboratoire de Nanotechnologie et d'Instrumentation Optique, Institute Charles Delaunay, Université de Technologie de Troyes, UMR-STMR CNRS, France.

Analytical Chemistry
|August 17, 2012
PubMed
Summary
This summary is machine-generated.

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Two new methods create gold nanostructures with tunable localized surface plasmon resonance (LSPR) on a single substrate. These gold nanostructures are used to develop sensitive immunosensors for detecting the atrazine herbicide.

Area of Science:

  • Nanotechnology
  • Materials Science
  • Surface Chemistry

Background:

  • Localized Surface Plasmon Resonance (LSPR) is crucial for optical sensing applications.
  • Controlling gold nanostructure properties is key to optimizing LSPR-based sensors.
  • Fabricating substrates with spatially controlled nanostructures presents a significant challenge.

Purpose of the Study:

  • To develop novel, efficient protocols for creating gold nanostructures with tunable LSPR properties on a single glass substrate.
  • To investigate the relationship between gold film thickness, nanoparticle characteristics, and LSPR response.
  • To demonstrate the utility of these tailored substrates in constructing highly sensitive LSPR immunosensors.

Main Methods:

  • Utilized vacuum evaporation and high-temperature annealing (550 °C) to fabricate gold nanostructures.

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Published on: March 17, 2023

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  • Implemented two distinct protocols: (1) metal masking for "shadowing effect" to vary film thickness, and (2) multi-cycle evaporation for predefined areas.
  • Characterized nanostructures using transmission UV-vis extinction spectroscopy and scanning electron microscopy (SEM).

    • Main Results:

    • Successfully modulated gold film thickness, leading to distinct zones of localized surface plasmon resonance (LSPR) gold nanostructures.
    • Protocol 1 enabled rapid screening of LSPR responses across different (bio)functionalized zones on a single substrate.
    • Protocol 2 demonstrated a strong correlation between evaporated film thickness, nanoparticle size, and plasmonic properties (resonant wavelength and peak amplitude).

    Conclusions:

    • Developed two versatile protocols for fabricating gold nanostructures with controlled LSPR properties.
    • The fabricated substrates are effective for rapid screening and optimization of LSPR sensor designs.
    • Successfully applied these substrates in the development of LSPR immunosensors for sensitive atrazine herbicide detection.