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Updated: May 19, 2026
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated ampC β-lactamase genes
Chelsie N Geyer1, Mark D Reisbig, Nancy D Hanson
1Creighton University School of Medicine Department of Medical Microbiology and Immunology Center for Research in Anti-Infectives and Biotechnology, Omaha, Nebraska, USA.
Abstract:
A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.
