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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: May 19, 2026

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins
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Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

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Patch-clamp protocols to study cell ionic homeostasis under saline conditions.

Ana María Velarde-Buendía1, René Alberto Enríquez-Figueroa, Igor Pottosin

  • 1Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima, Mexico.

Methods in Molecular Biology (Clifton, N.J.)
|August 17, 2012
PubMed
Summary
This summary is machine-generated.

The patch-clamp technique measures ion transport across cell membranes. This study details methods for assessing potassium, cation, anion channels, and proton pumps crucial for cellular salt balance.

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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Published on: October 1, 2010

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Last Updated: May 19, 2026

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Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
15:28

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Published on: October 1, 2010

Area of Science:

  • Plant physiology
  • Cell biology
  • Biophysics

Background:

  • Intracellular ionic homeostasis is vital for plant survival, especially under saline conditions.
  • Electrogenic transport across cellular membranes plays a critical role in maintaining this balance.
  • The patch-clamp technique is a powerful tool for studying membrane transport proteins.

Purpose of the Study:

  • To describe the preparation of protoplasts and vacuoles for electrophysiological studies.
  • To detail patch-clamp assay protocols for analyzing ion channel and pump activity.
  • To demonstrate the detection of key ion transporters involved in saline stress response.

Main Methods:

  • Isolation and preparation of plant protoplasts and vacuoles.
  • Application of the patch-clamp technique to measure membrane currents.
  • Functional analysis of potassium (K+) and cation channels, anion channels, and proton (H+) pumps in plasma and tonoplast membranes.

Main Results:

  • Successful preparation of protoplasts and vacuoles enabling electrophysiological measurements.
  • Detection of functional K+ and cation channels in plasma membranes and tonoplast.
  • Identification of plasma membrane anion channels and vacuolar/plasma membrane H+ pumps contributing to ion homeostasis.

Conclusions:

  • The described patch-clamp methods are effective for characterizing ion transport proteins in plant cells.
  • These transporters are essential for maintaining intracellular ionic homeostasis under saline stress.
  • This work provides a foundation for further research into salt tolerance mechanisms in plants.