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Updated: May 19, 2026
Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
Published on: October 4, 2012
Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis
Aurelie Evrard1, Bastiaan O R Bargmann, Kenneth D Birnbaum
1Australian Centre for Plant Functional Genomics, University of Adelaide, Glen Osmond, SA, Australia.
Abstract:
Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.
