Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Types of Electrodes01:19

Potentiometry: Types of Electrodes

Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
An alternative to SHE is the Saturated Calomel Electrode (SCE). This electrode features an...
Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential ensures...
Standard Electrode Potentials03:02

Standard Electrode Potentials

On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
Calibration Curves: Linear Least Squares01:20

Calibration Curves: Linear Least Squares

A calibration curve is a plot of the instrument's response against a series of known concentrations of a substance. This curve is used to set the instrument response levels, using the substance and its concentrations as standards. Alternatively, or additionally, an equation is fitted to the calibration curve plot and subsequently used to calculate the unknown concentrations of other samples reliably.
For data that follow a straight line, the standard method for fitting is the linear...
Coulometry: Overview01:00

Coulometry: Overview

Coulometry is one of the rapid, most accurate, and precise analytical techniques that determine the quantity of an analyte by measuring the electrical charge needed for its complete electrolysis without using any analytical standards. The total charge passed during electrolysis correlates with the analyte amount by Faraday's laws of electrolysis. For accurate coulometric measurements, a charge equal to Faraday's constant multiplied by the number of electrons involved in the relevant...
Interfacial Electrochemical Methods: Overview01:06

Interfacial Electrochemical Methods: Overview

Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current passing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fatal pediatric collapse with bilateral subdural hematoma and retinal hemorrhage occurring in a public park: a forensic case study.

Journal of forensic and legal medicine·2026
Same author

Determinants of diaphragm ultrasound and its diagnostic performance for predicting respiratory status in myotonic dystrophy type 1.

Respiratory medicine and research·2026
Same author

Laminopathies: natural history and risk prediction of heart failure.

European heart journal·2026
Same author

Prognostic value of right ventricular-pulmonary artery coupling in patients with muscular dystrophies.

Scientific reports·2026
Same author

A critical review of the American Academy of Pediatrics technical report on abusive head trauma.

Forensic science international. Synergy·2025
Same author

Electrocardiogram vs Electrophysiological Study and Major Conduction Delays in Myotonic Dystrophy Type 1.

JAMA cardiology·2025
Same journal

Targeting intracranial electrical stimulation to network regions defined within individuals causes network-level effects.

Journal of neurophysiology·2026
Same journal

When "Noise" Isn't Simply Noise: Deterministic Postural Drive During Noisy Galvanic Vestibular Stimulation (nGVS).

Journal of neurophysiology·2026
Same journal

Abrupt Scene Onsets and Gradually Emerging Scene Information Produce Distinct EEG Decoding Dynamics.

Journal of neurophysiology·2026
Same journal

From discovery to translation: charting a course for the <i>Journal of Neurophysiology</i>.

Journal of neurophysiology·2026
Same journal

Neuromodulatory Strategies Overcome Multiple Inevitable Impairments of Cerebral Palsy.

Journal of neurophysiology·2026
Same journal

Acute Fentanyl Toxicity:From Opioid-Induced to Hypoxia-Mediated Pathophysiology.

Journal of neurophysiology·2026
See all related articles

Related Experiment Video

Updated: May 19, 2026

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

A calibration-free electrode compensation method.

Cyrille Rossant1, Bertrand Fontaine, Anna K Magnusson

  • 1Laboratoire Psychologie de la Perception, Centre National de la Recherche Scientifique, Université Paris Descartes, Paris, France.

Journal of Neurophysiology
|August 17, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a novel offline method to accurately compensate for electrode artifacts in electrophysiology recordings without prior calibration. This technique improves neuronal data quality by tracking electrode properties over time.

More Related Videos

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
08:03

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Published on: April 18, 2013

Related Experiment Videos

Last Updated: May 19, 2026

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
08:03

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Published on: April 18, 2013

Area of Science:

  • Neuroscience
  • Electrophysiology
  • Biophysics

Background:

  • Single-electrode current-clamp recordings measure both neuronal membrane and electrode potentials.
  • Traditional bridge balance methods for electrode compensation introduce capacitive transients due to non-ideal electrode resistance.
  • Existing advanced methods require preliminary electrode calibration, which can lead to corrupted data if electrode properties change.

Purpose of the Study:

  • To develop a new offline electrode compensation method that eliminates the need for preliminary calibration.
  • To improve the accuracy and reliability of electrophysiological recordings by addressing electrode artifacts.
  • To enable tracking of electrode properties over time during recordings.

Main Methods:

  • Proposed an offline compensation technique involving fitting a neuron-electrode model to the recorded trace.
  • Subtracted the predicted electrode response from the measurement.
  • Utilized an error criterion designed to prevent distortion of compensated traces by neuronal spikes.
  • Demonstrated the method with biophysical models and whole-cell recordings in cortical and brain-stem neurons.

Main Results:

  • The proposed method effectively compensates for electrode responses without prior calibration.
  • The technique avoids capacitive transients often introduced by traditional bridge balance.
  • Electrode properties can be dynamically tracked over the course of recordings.
  • The method shows successful application in both simulated data and experimental recordings.

Conclusions:

  • The novel offline compensation method offers a robust alternative to traditional techniques for electrophysiological recordings.
  • This approach enhances data integrity by accurately removing electrode artifacts and allowing for dynamic property tracking.
  • The technique is versatile and can be extended to various electrode and neuronal models.