Mineral concentrations of plasma and liver after injection with a trace mineral complex differ among Angus and...

To examine the effects of cattle breed on the clearance rate of an injectable mineral product, 10 Angus and 10 Simmental steers were blocked by breed and initial BW (332 ± 33 kg) and injected with either Multimin 90 (MM) or sterilized saline (CON) at a dose of 1 mL/45 kg BW. Multimin 90 contains 15 mg Cu/mL (as Cu disodium EDTA), 60 mg Zn/mL (as Zn disodium EDTA), 10 mg Mn/mL (as Mn disodium EDTA), and 5 mg Se/mL (as sodium selenite). Steers received a corn-silage-based diet, and inorganic sources of Cu, Zn, Mn, and Se were supplemented at NRC recommended amounts. Jugular blood was collected immediately before injection and at 8 and 10 h post-injection and on days 1, 8, and 15 post-injection. Liver biopsies were collected 3 d before injection and on days 1, 8, and 15 post-injection. Liver and plasma mineral concentration and glutathione peroxidase (GSH-Px) activity data were analyzed as repeated measures. Plasma concentrations of Zn, Mn, and Se were greater (P = 0.01) and Cu tended to be greater (P = 0.12) post-injection in MM steers compared with the CON steers. Regardless of treatment, Simmental cattle had lower plasma concentrations of Cu, Zn, and Se (P ≤ 0.05) when compared with Angus cattle. Erythrocyte GSH-Px activity was greater (P = 0.01) in MM steers compared with CON steers. Liver concentrations of Cu, Zn, and Se were greater (P = 0.05) in MM steers compared with CON steers post-injection. Liver Mn concentrations tended to be greater (P = 0.06) in MM steers compared with CON steers in the days post-injection. Interestingly, Simmental cattle exhibited greater (P = 0.01) liver Mn concentrations in the days after injection compared with Angus cattle (7.0 and 6.0 mg Mn/kg for Simmental and Angus cattle, respectively), regardless of treatment. It is unclear if this breed difference is biologically relevant; however, these data may suggest that differences in liver excretion of Mn exist between the two breeds. Overall, use of an injectable trace mineral increased liver concentrations of Cu and Se through the 15-d sampling period, suggesting that this injectable mineral is an adequate way to improve Cu and Se status of cattle through at least 15 d.