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Multicellular organisms employ a variety of ways for cells to communicate with each other. Gap junctions are specialized proteins that form pores between neighboring cells in animals, connecting the cytoplasm between the two, and allowing for the exchange of molecules and ions. They are found in a wide range of invertebrate and vertebrate species, mediate numerous functions including cell differentiation and development, and are associated with numerous human diseases, including cardiac and...

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Related Experiment Video

Updated: May 19, 2026

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

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Published on: July 16, 2013

Gentamicin affects connexin 26 expression in the cochlear lateral wall.

P Hu¹, R Lai, D Xie

¹Institute of Otology, Department of Otolaryngology and Head and Neck surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.

B-ENT

|August 18, 2012

Summary

Gentamicin, an aminoglycoside antibiotic, increases connexin 26 expression in rat cochlear fibrocytes. This suggests connexin 26 is involved in aminoglycoside-induced ototoxicity and acquired hearing loss.

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Last Updated: May 19, 2026

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Published on: January 9, 2019

Area of Science:

Ototoxicity research
Molecular biology of hearing
Drug-induced hearing loss

Background:

Aminoglycosides can alter ion-exchange proteins in the cochlea.
Connexin 26 is crucial for maintaining the endocochlear potential and endolymph potassium levels.
Dysregulation of connexin 26 may contribute to acquired hearing loss.

Purpose of the Study:

To investigate the impact of gentamicin on connexin 26 expression.
To elucidate the role of connexin 26 in aminoglycoside ototoxicity.

Main Methods:

Rats received a single dose of gentamicin.
Connexin 26 protein and mRNA levels were analyzed in the cochlear lateral wall.
Techniques included immunohistochemistry, western blotting, and real-time PCR.

Main Results:

Connexin 26 expression increased in type III fibrocytes post-gentamicin.
Elevated protein levels were observed 3 hours after gentamicin injection.
Increased mRNA levels were detected 24 hours after gentamicin administration.

Conclusions:

Connexin 26 is implicated in the acute effects of high-dose gentamicin.
Connexin 26 likely plays a role in the pathogenesis of ototoxic deafness.