Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 19, 2026

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

Human brown fat inducible thioesterase variant 2 cellular localization and catalytic function.

Danqi Chen1, John Latham, Hong Zhao

  • 1Department of Chemistry and Chemical Biology, University of New Mexico , Albuquerque, NM 87131, USA.

Biochemistry
|August 18, 2012
PubMed
Summary

Brown fat inducible thioesterase variant 2 (BFIT2) is a mitochondrial protein with thioesterase activity. Its StarD14 domain binds phosphatidylinositol 4-phosphate, differentiating its function from its cytoplasmic paralog.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MiR-32-3p improves ISO induced AC16 myocardial cell injury by targeting ATF4.

Journal of cardiothoracic surgery·2026
Same author

Structure-guided discovery of 1H-pyrazolo[3,4-c]pyridin-3-amines as a novel class of hematopoietic progenitor kinase 1 (HPK1) inhibitors.

European journal of medicinal chemistry·2026
Same author

Ultrasound-Guided Superior Laryngeal Nerve Block Combined with Cricothyroid Membrane Puncture for Awake Tracheal Intubation in a Patient with a Laryngeal Tumor: A Case Report and Literature Review.

International medical case reports journal·2026
Same author

High-Flow Nasal Cannula Oxygen Therapy in Perioperative Respiratory Care: Application and Prospects.

Journal of multidisciplinary healthcare·2026
Same author

Applications of KinTek Explorer Kinetic Simulations of Targeted Protein Degradation for Evaluation and Design of PROTACs.

Biochemistry·2025
Same author

Corrigendum to "Formaldehyde induces and promotes Alzheimer's disease pathologies in a 3D human neural cell culture model" [Food Chem. Toxicol. (2025 Dec) 206 115777].

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2025

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Brown fat inducible thioesterase variant 2 (BFIT2), or ACOT11, is a protein with hotdog-fold and StarD14 domains.
  • Understanding BFIT2's localization and function is crucial for metabolic research.

Purpose of the Study:

  • To determine the subcellular localization and catalytic activity of human BFIT2 (hBFIT2).
  • To investigate the specific lipid-binding properties of the hBFIT2 StarD14 domain.

Main Methods:

  • HEK293 cell transfection and protein isolation.
  • In vitro thioesterase activity assays.
  • Protein-lipid overlay assays.

Main Results:

  • The N-terminus of hBFIT2 acts as a mitochondrial signal sequence, leading to its localization in the mitochondrial matrix after cleavage.

More Related Videos

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
08:31

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

Published on: July 1, 2021

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
07:22

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

Related Experiment Videos

Last Updated: May 19, 2026

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
08:31

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

Published on: July 1, 2021

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
07:22

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

  • Mature hBFIT2 exhibits selective thioesterase activity towards long-chain fatty acyl-CoA thioesters.
  • The StarD14 domain of hBFIT2 specifically binds phosphatidylinositol 4-phosphate.

    • Conclusions:

    • hBFIT2 is a mitochondrion-localized enzyme with distinct thioesterase activity compared to its cytoplasmic paralog, CACH (ACOT12).
    • The StarD14 domain's interaction with phosphatidylinositol 4-phosphate suggests a role in specific lipid metabolism pathways within mitochondria.