Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting immune cells in the aged brain reveals that engineered cytokine IL-10 enhances neurogenesis and improves cognition.

Immunity·2026
Same author

Design principles of a membrane-spanning ubiquitin ligase.

Molecular cell·2026
Same author

A dual-pathway Wnt-IL-13 fusion protein enhances human intestinal regeneration through tuft cell activation.

The Journal of biological chemistry·2026
Same author

Deep peptide recognition profiling decodes TCR specificity and enables disease-associated antigen discovery.

Nature biotechnology·2026
Same author

Viral vector-free generation of orthogonal IL-2-responsive CAR T cells through gene editing of IL-2 and its receptor.

Blood immunology & cellular therapy·2026
Same author

Intratumoral T<sub>reg</sub> cell ablation elicits NK cell-mediated control of CD8 T cell-resistant tumors.

Science immunology·2026
Same journal

An Essential Role for Senescent Cells in Optimal Wound Healing through Secretion of PDGF-AA.

Developmental cell·2026
Same journal

AXIN1 and AXIN2 regulate the WNT-signaling landscape to promote distinct mesoderm programs.

Developmental cell·2026
Same journal

ARID1A terminates gastric regeneration to prevent cancer.

Developmental cell·2026
Same journal

Myc sustains sex-biased organ zonation in the Drosophila intestine.

Developmental cell·2026
Same journal

Two parallel neuronal circuits involving electrical synapse and DAF-7/TGF-β signaling regulate muscle autophagy in C. elegans.

Developmental cell·2026
Same journal

Menstruation: Once unspoken but now uncovered, one cell type at a time.

Developmental cell·2026
See all related articles

Related Experiment Video

Updated: May 19, 2026

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Structural architecture and functional evolution of Wnts.

J Fernando Bazan1, Claudia Y Janda, K Christopher Garcia

  • 1Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA. jfbazan@gmail.com

Developmental Cell
|August 18, 2012
PubMed
Summary
This summary is machine-generated.

The Wnt-Frizzled complex structure reveals evolutionary origins and explains dual lipid-protein interactions for Wnt signaling. This finding clarifies how Wnt proteins bind to Frizzled receptors.

More Related Videos

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

Related Experiment Videos

Last Updated: May 19, 2026

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

Area of Science:

  • Structural Biology
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Wnt proteins are crucial signaling molecules involved in development and disease.
  • Frizzled receptors are key mediators of Wnt signaling pathways.
  • The precise interaction mechanism between Wnts and Frizzleds remains incompletely understood.

Purpose of the Study:

  • To elucidate the structural basis of Wnt morphogen binding to the Frizzled receptor ectodomain.
  • To understand the evolutionary history of the Wnt-Frizzled complex structure.
  • To explain the dual mode of Wnt engagement with Frizzled receptors.

Main Methods:

  • X-ray crystallography to determine the high-resolution structure of the Wnt-Frizzled complex.
  • Bioinformatic analyses to infer evolutionary relationships.
  • Biochemical assays to validate interaction interfaces.

Main Results:

  • The crystal structure reveals intricate details of the Wnt-Frizzled ectodomain interface.
  • The fold of the complex shows conserved features suggesting deep evolutionary origins.
  • Specific lipid and protein contacts mediating Wnt binding to Frizzled were identified.

Conclusions:

  • The determined structure provides a molecular framework for Wnt-Frizzled interactions.
  • Understanding these interactions offers insights into Wnt pathway regulation.
  • This structural knowledge can inform therapeutic strategies targeting Wnt signaling.