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Related Experiment Video

Updated: May 19, 2026

Single-cell Microinjection for Cell Communication Analysis
09:59

Single-cell Microinjection for Cell Communication Analysis

Published on: February 26, 2017

Voltage controlled nano-injection system for single-cell surgery.

R Adam Seger1, Paolo Actis, Catherine Penfold

  • 1Department of Biomolecular Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA.

Nanoscale
|August 18, 2012
PubMed
Summary
This summary is machine-generated.

We developed a novel single-cell injection platform using nanopipettes for precise molecular delivery. This technology enables advanced manipulation and analysis of individual cells, crucial for understanding cell function and fate.

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Last Updated: May 19, 2026

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Area of Science:

  • Cell biology
  • Biotechnology
  • Microfluidics

Background:

  • Understanding cellular processes requires precise manipulation of individual cells.
  • Current methods for single-cell analysis face limitations in targeted molecular delivery.

Purpose of the Study:

  • To introduce a new platform for single-cell injection using nanopipettes.
  • To demonstrate the capability of delivering molecules into individual cells with high precision.

Main Methods:

  • Development of a single-cell injection system utilizing nanopipettes.
  • Integration of scanning microscopy for cell surface detection.
  • Application of voltage pulses for controlled molecule delivery into cells.

Main Results:

  • Successfully demonstrated the functionality of the nanopipette-based injection platform.
  • Achieved precise delivery of molecules into adherent mammalian cells.
  • Validated the system through injection of fluorescent dyes into single cells.

Conclusions:

  • The nanopipette platform offers a powerful tool for single-cell manipulation and analysis.
  • This technology advances the study of cellular functions and cell fate determination.
  • Potential applications in various fields of cell biology and drug delivery research.