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Hyaluronan and collagen in human hypertrophic cardiomyopathy: a morphological analysis

Martin Hellström1, Anna Engström-Laurent, Stellan Mörner

  • 1Department of Public Health and Clinical Medicine, Medicine, Umeå University, 90187 Umeå, Sweden.

Cardiology Research and Practice
|August 18, 2012
PubMed

Insights

Hyaluronan (HA) is more abundant in hypertrophic cardiomyopathy (HCM) hearts, but its receptor CD44 is not detected. HA and collagen I share similar locations in HCM myocardial tissue.

Area of Science:

  • Cardiovascular Pathology
  • Extracellular Matrix Biology

Background:

  • Hypertrophic cardiomyopathy (HCM) involves cardiomyocyte and extracellular matrix (ECM) abnormalities.
  • Hyaluronan (HA) and its receptor CD44 are implicated in cell growth and proliferation.
  • The role of HA and its distribution in HCM, particularly in relation to collagen, is not well understood.

Purpose of the Study:

  • To investigate the histological distribution of hyaluronan (HA) in human hypertrophic cardiomyopathy (HCM).
  • To examine the presence of the HA receptor, CD44, in HCM myocardium.
  • To compare the localization of HA and collagen I in HCM.

Main Methods:

  • Myocardial tissue samples were obtained from 5 patients undergoing myectomy for symptomatic HCM.
  • Histochemistry and immunohistochemistry techniques were employed to analyze tissue composition.
  • Staining for HA, CD44, and Collagen I was performed on patient and control samples.

Main Results:

  • Hyaluronan (HA) staining intensity was significantly higher in HCM patients compared to controls.
  • HA was distributed interstitially, within fibrous septa, and in the adventitia of intramyocardial vessels in both groups.
  • CD44 receptor was not detected in the myocardium of either HCM patients or controls.
  • Collagen I exhibited a similar overall localization to HA, but with distinct detailed distribution patterns.

Conclusions:

  • This study provides the first description of hyaluronan distribution in human HCM.
  • Increased HA staining in HCM is not associated with CD44 receptor coexpression in the chronic phase.
  • Hyaluronan and Collagen I share common localization patterns within the hypertrophic cardiomyopathy myocardium.

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