Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Blood Transfusion and Agglutination02:45

Blood Transfusion and Agglutination

Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
Transcytosis of IgG01:15

Transcytosis of IgG

Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
Composition of Blood Plasma01:24

Composition of Blood Plasma

Blood plasma is a fluid that contains approximately 92% water and 8% solutes. The solutes include various types of proteins, which constitute about 7% of the total solutes in the plasma. The high-molecular-weight proteins—albumins, globulins, and fibrinogen—are essential to plasma function. Albumins, making up about 60% of the plasma proteins, maintain the osmotic balance within blood vessels by preventing excessive water leakage. Additionally, albumins serve as carrier proteins, binding to...
Humoral Immune Responses01:36

Humoral Immune Responses

Overview
Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Protein Buffers in Blood Plasma and Cells01:20

Protein Buffers in Blood Plasma and Cells

The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional Diversity of the Excretory/Secretory Proteins of Nematode Parasites.

Acta parasitologica·2022
Same author

Enhancing the Stability of Haemonchus contortus Glyceraldehyde-3-Phosphate Dehydrogenase and Binding of Host Albumin to the Parasite Enzyme.

Acta parasitologica·2020
Same author

Complement C9 binding site and the anti-microbial activity of caprine vitronectin are localized in close proximity in the N-terminal region of the protein.

Microbial pathogenesis·2020
Same author

Staphylococcus aureus autolysins interact with caprine vitronectin without involving the heparin binding domain and the second arginine-glycine-aspartic acid motif of the host protein.

Archives of microbiology·2019
Same author

Defining the complement C3 binding site and the antigenic region of Haemonchus contortus GAPDH.

Parasite immunology·2018
Same author

Conjugation of ampicillin and enrofloxacin residues with bovine serum albumin and raising of polyclonal antibodies against them.

Veterinary world·2016

Related Experiment Video

Updated: May 19, 2026

Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
10:55

Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance

Published on: October 16, 2018

Characterization of goat plasma vitronectin.

S Suchitra1, Vasili Ashok, Tapas Gupta

  • 1Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar (U.P.) 243 122, India.

Indian Journal of Biochemistry & Biophysics
|August 21, 2012
PubMed
Summary

Goat vitronectin (VN) was isolated and characterized. The 81 kDa form exhibits cell adhesion and heparin binding, suggesting it is the monomeric unit of VN.

More Related Videos

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy
08:07

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy

Published on: January 12, 2024

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery
05:30

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

Published on: January 31, 2025

Related Experiment Videos

Last Updated: May 19, 2026

Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
10:55

Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance

Published on: October 16, 2018

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy
08:07

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy

Published on: January 12, 2024

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery
05:30

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

Published on: January 31, 2025

Area of Science:

  • Biochemistry
  • Cell Biology

Background:

  • Vitronectin (VN) is a key extracellular matrix protein involved in cell adhesion and migration.
  • Understanding VN's structure-function relationship is crucial for various biological processes.

Purpose of the Study:

  • To isolate and characterize vitronectin (VN) from goat plasma in native and denatured states.
  • To investigate the structural properties and functional activities of different VN forms.

Main Methods:

  • Isolation and characterization of VN from goat plasma.
  • SDS-gel electrophoresis to determine molecular weights.
  • Cell adhesion assays to assess biological activity.
  • Heparin binding assays.

Main Results:

  • Native VN comprised 160 and >250 kDa polypeptides; denatured VN showed 81 and >250 kDa bands.
  • The 81 kDa polypeptide, upon storage, formed higher molecular weight forms, suggesting it's the monomer.
  • Both native and denatured VN exhibited cell adhesive properties, with distinct cell morphologies observed.
  • The 81 kDa VN bound to heparin, while the 160 kDa form did not in the presence of urea.
  • Goat VN degraded in the absence of EDTA and upon addition of excess Ca(2+), indicating metalloprotease activity.

Conclusions:

  • The 81 kDa polypeptide is likely the monomeric unit of goat VN, forming dimers and multimers.
  • Goat VN exhibits cell adhesive properties in both native and denatured states.
  • The 81 kDa VN possesses heparin-binding capabilities, and goat VN is susceptible to metalloprotease degradation.