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Published on: November 10, 2021
Purification and partial characterization of cytochrome c552 from Halobacterium salinarium
1Department of Biochemistry, Gulbarga University Gulbarga-585 106, Karnataka, India. ksramu@rediffmail.com
Indian Journal of Biochemistry & Biophysics
|August 21, 2012
Abstract:
Cytochrome c552 was purified to near homogenity and partially characterized from Halobacterium salinarium JWS mutant, devoid of carotenoid pigments. The purification involved the extraction of membranes with 1% Triton X-100, followed by butylagarose, DEAE-Sepharose CL6B and hydroxyapatite column chromatography. The fold of purification was 16. The purified cytochrome showed maximum absorption at 552 nm. The molecular mass determined by SDS-PAGE was found to be 14.1 kD.