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Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer
Published on: November 4, 2009
Microwave-assisted method for microassay of proteins using bicinchoninic acid
Sulakshana Jain1, Shweta Sharma, Munishwar N Gupta
1Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Indian Journal of Biochemistry & Biophysics
|August 21, 2012
Abstract:
A microwave-assisted microassay has been developed for estimation of proteins, using bicinchoninic acid (BCA). The protocol described here is rapid with a reaction time of merely 95 sec, requires lesser amounts of BCA and other reagents, and tolerates larger or comparable concentration of various interfering substances.
