Purification of catecholase from Solanum melangena (brinjal)

Catecholase was purified from cortex of Solanum melangena (brinjal) on natural affiant, lignin. The elution profile showed seven peaks with the 6th peak having 4616-fold purity. The 6th pure fraction loaded on PAGE showed two protein bands on staining with Coomassie brilliant blue, one at the point of application and other near the dye front. These bands exhibited catecholase activity, when stained with 4-methyl catechol and proline, Basic fuschin and ethidium bromide showed positive tests, indicating that catecholase is a ribonucleoglycoprotein.