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Related Experiment Video

Updated: May 19, 2026

In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells

In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells

Published on: May 2, 2008

Molecular live cell bioimaging in stem cell research.

Max Endele¹, Timm Schroeder

¹Research Unit Stem Cell Dynamics, Helmholtz Center Munich-German Research Center for Environmental Health, Neuherberg, Germany.

Annals of the New York Academy of Sciences

|August 21, 2012

Summary

Live cell imaging with fluorescent biosensors reveals cellular heterogeneity and signaling dynamics, linking them to future cell fate decisions for improved biological understanding.

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Super-Resolution Live Cell Imaging of Subcellular Structures

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Published on: January 13, 2021

Area of Science:

Cell Biology
Biotechnology
Systems Biology

Background:

Functional heterogeneity in stem and progenitor cells impacts cell fate.
Intracellular signaling heterogeneity, in spatiotemporal activity, influences cell behavior.

Purpose of the Study:

To quantify heterogeneous cellular states and link them to future cell fates.
To highlight the utility of live cell imaging and biosensors for studying cellular heterogeneity.

Main Methods:

Live cell imaging techniques.
Fluorescent biosensors for monitoring intracellular signaling activity.
Single-cell resolution observation of cell populations.

Main Results:

Live cell imaging combined with biosensors enables continuous observation of cellular and molecular heterogeneity.
This approach allows for the study of long-term biological effects of signaling.
Methodologies offer advantages over classical approaches for understanding signaling responses.

Conclusions:

Live cell imaging and biosensors are powerful tools for dissecting cellular and molecular heterogeneity.
These methods can elucidate the role of heterogeneous responses in cell fate decisions.
Application of these techniques can advance our understanding of signaling dynamics and cell behavior.