Complete sequence of the suicide vector pJP5603
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Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
Published on: October 31, 2019
Thomas Riedel1, Meike Rohlfs, Ina Buchholz
1Helmholtz-Centre for Infection Research, Group Microbial Communication, Braunschweig, Germany. tri07@helmholtz-hzi.de
Plasmid
|August 21, 2012
Abstract:
We have sequenced the complete R6K-based and mobilizable suicide vector pJP5603. For the replication of the vector a trans supply of the pir-encoded π protein of plasmid R6K is essential. The 3.126 kb plasmid encodes a kanamycin resistance cassette for selection and contains a lacZ-α-system that allows a blue-white selection of cloned fragments.
