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Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...

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Related Experiment Video

Updated: May 19, 2026

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
07:13

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Published on: April 26, 2011

Nucleosomes affect local transformation efficiency.

Elham Aslankoohi1, Karin Voordeckers, Hong Sun

  • 1Laboratory for Systems Biology, VIB, Bio-Incubator, Gaston Geenslaan 1, B-3001 Leuven, Belgium.

Nucleic Acids Research
|August 21, 2012
PubMed
Summary
This summary is machine-generated.

Nucleosomes, DNA packaging structures, inversely correlate with genetic transformation efficiency in yeast. Understanding nucleosome positioning helps predict and improve DNA integration success in recombinant gene technology.

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Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

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Related Experiment Videos

Last Updated: May 19, 2026

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
07:13

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Published on: April 26, 2011

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy
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In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

Published on: September 6, 2024

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
09:26

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Published on: March 26, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Genetic transformation is crucial for natural horizontal gene transfer and modern recombinant gene technology.
  • Factors influencing transformation efficiency are not fully understood.
  • Nucleosome positioning, affecting DNA accessibility, is a potential determinant of transformation efficiency.

Purpose of the Study:

  • To investigate the relationship between nucleosome positioning and local transformation efficiency in Saccharomyces cerevisiae.
  • To determine if DNA accessibility, influenced by nucleosomes, impacts the success rate of genetic transformation.

Main Methods:

  • Mapping transformation efficiency across various genomic locations in Saccharomyces cerevisiae.
  • Correlating transformation efficiency data with genome-wide nucleosome positioning data.
  • Analyzing the impact of altered nucleosome patterns on transformation efficiency.

Main Results:

  • A significant inverse correlation was observed between transformation efficiency and relative nucleosome density.
  • This correlation was abolished when the nucleosome pattern was altered, irrespective of the underlying DNA sequence.
  • Nucleosome positioning, not DNA sequence alone, dictates local transformation efficiency.

Conclusions:

  • Nucleosomes play a significant role in regulating genetic transformation efficiency.
  • The findings provide a method to predict transformation efficiency based on genomic location.
  • This research enables the selection of genomic regions with higher transformation potential for improved gene technology applications.