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Video-rate Scanning Confocal Microscopy and Microendoscopy

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

A line scanning confocal fluorescent microscope using a CMOS rolling shutter as an adjustable aperture.

E Mei¹, P A Fomitchov, R Graves

¹GE Healthcare, Life Sciences Division, Piscataway, NJ 08854, USA. erwen.mei@ge.com

Journal of Microscopy

|August 22, 2012

Summary

This study introduces a novel line scanning confocal microscope using a software-controlled rolling shutter instead of a physical aperture. This innovation allows for real-time, adjustable confocal resolution for improved imaging in microscopy applications.

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Area of Science:

Microscopy and Imaging Technologies
Optical Physics

Background:

Traditional confocal microscopy relies on physical aperture barriers, limiting confocality control.
The fixed nature of physical apertures restricts real-time adjustments to imaging parameters.

Purpose of the Study:

To present a new line scanning confocal microscope design that replaces physical apertures with a software-controllable rolling shutter.
To demonstrate adjustable and independent confocal resolution per fluorescence channel.

Main Methods:

Implementation of a rolling shutter on a CMOS camera synchronized with laser line scanning.
Synchronization of rolling shutter width with laser scanning for image acquisition.
Real-time adjustment of confocal resolution by modifying rolling shutter width.

Main Results:

Successful elimination of the need for a physical aperture in confocal microscopy.
Demonstration of adjustable confocal resolution in real time.
Independent control of resolution for each fluorescence channel.

Conclusions:

The developed line scanning confocal microscope offers enhanced flexibility and control over confocality.
This technology enables adjustable resolution, improving imaging capabilities for various applications.
The system has been successfully implemented in the GE Healthcare IN Cell Analyzer 6000.