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Updated: May 19, 2026

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
Published on: October 15, 2016
Production of antibody fragments in Escherichia coli
Tomohisa Katsuda1, Hiroyuki Sonoda, Yoichi Kumada
1Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan. katsuda@kobe-u.ac.jp
Abstract:
Escherichia coli is a host widely used in the industrial production of recombinant proteins. However, the expression of heterologous proteins in E. coli often encounters the formation of inclusion bodies, which are insoluble and nonfunctional protein aggregates. For the successful production of antibody fragments, which includes single-chain variable fragments (scFvs), we describe here the modification of linker, signal, and Shine-Dalgarno (SD) sequences, the coexpression of cytoplasmic and periplasmic chaperones, and a method for fed-batch cultivation with exponential feed.

