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Updated: May 19, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
Production of antibody derivatives in the methylotrophic yeast Pichia pastoris
Steve Schoonooghe1, Jannick Leoen, Jurgen Haustraete
1Department of Cellular and Molecular Immunology, Vrije Universiteit Brussel (CMIM-VUB), Brussels, Belgium. sschoono@vub.ac.be
Abstract:
New antibody derivatives are continuously being generated to interact with a range of therapeutic targets. The cost-effective and efficient production of these and other antibody derivatives is crucial for their further success. Here, we describe the construction of the expression vectors needed for heterologous expression of a Fab fragment in the yeast Pichia pastoris. The experimental conditions for lab-scale expressions are discussed, and an overview of an efficient purification strategy is presented.
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