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Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
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Selection and use of intracellular antibodies (intrabodies)

Sandrine Moutel1, Clément Nizak, Franck Perez

  • 1Institut Curie, CNRS UMR144, Paris, France.

Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2012
PubMed

Intracellularly expressed recombinant antibodies, or intrabodies, are powerful tools for cell biology studies as well as therapeutic applications. Cell biologists use them to either block the intracellular antibody target or to image endogenous target dynamics. We describe here methods to select recombinant antibodies from antibody phage display libraries and to subsequently express them as fluorescent intrabodies.

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