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Related Experiment Video

Updated: May 19, 2026

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Published on: April 22, 2022

Specialized technologies for epigenetics in plants.

¹Department of Biology, Saint Louis University, St. Louis, MO, USA. wxiao@slu.edu

Methods in Molecular Biology (Clifton, N.J.)

|August 22, 2012

Summary

Plant imprinting, essential for reproduction, involves differential gene expression from maternal or paternal alleles. This study details methods to analyze gene imprinting and DNA methylation in Arabidopsis endosperm.

Area of Science:

Plant epigenetics
Reproductive biology
Molecular genetics

Background:

Imprinting, the parent-specific gene expression, is crucial for plant and mammal reproduction.
DNA methylation patterns dictate the silencing or expression of imprinted genes in plants.
Endosperm, the tissue formed after double fertilization in flowering plants, is a key site for imprinting.

Purpose of the Study:

To detail a protocol for analyzing gene imprinting and DNA methylation in Arabidopsis endosperm.
To provide methods for genetic crossing, endosperm isolation, and imprinting status determination.
To enable the analysis of DNA methylation in imprinted genes using bisulfite sequencing.

Main Methods:

Performing genetic crosses in Arabidopsis.

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Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Published on: June 7, 2013

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Last Updated: May 19, 2026

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Published on: April 22, 2022

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Published on: June 7, 2013

Isolating endosperm tissue from developing seeds.

Determining gene imprinting status.

Analyzing DNA methylation via bisulfite sequencing.

Main Results:

Established a comprehensive protocol for studying plant imprinting.
Identified key regulators of imprinting in Arabidopsis, including DNA METHYLTRANSFERASE1 (MET1) and DEMETER (DME).
Highlighted the role of Polycomb group proteins (PRC2) in regulating imprinted gene chromatin states.

Conclusions:

The protocol facilitates detailed investigation of epigenetic mechanisms in plant reproduction.
Understanding imprinting in endosperm is vital for plant development and reproductive success.
Further research is needed to elucidate mechanisms for maternally expressed genes regulated by unknown factors.