Related Experiment Video
Updated: May 19, 2026

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
Published on: April 3, 2015
CdGAP regulates cell migration and adhesion dynamics in two-and three-dimensional matrix environments
Duncan Wormer1, Nicholas O Deakin, Christopher E Turner
1Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA.
Abstract:
CdGAP is a Rac1/Cdc42 specific GTPase activating protein (GAP) that localizes to cell-matrix adhesions through an interaction with the adhesion scaffold α-parvin/actopaxin to regulate lamellipodia formation and cell spreading. Herein, we demonstrate, using a combination of siRNA-mediated silencing and overexpression, that cdGAP negatively regulates directed and random migration by controlling adhesion maturation and dynamics through the regulation of both adhesion assembly and disassembly. Interestingly, cdGAP was also localized to adhesions formed in three-dimensional (3D) matrix environments and cdGAP depletion promoted cancer cell migration and invasion through 3D matrices. These findings highlight the importance of GAP proteins in the regulation of Rho family GTPases and the coordination of the cell migration machinery..
Related Concept Videos
Overview of Cell-Matrix Interactions
Intracellular Signaling Affects Focal Adhesions
Some...
Cell Migration
Cell Migration
Cytoskeletal Coordination in Cell Migration
Cell-matrix's Response to Mechanical Forces
Anchoring junctions mechanically attach a cell to the...

