Related Experiment Video
Updated: May 19, 2026

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
Published on: April 26, 2024
Effect of fluoride dentifrices on the microhardness of deciduous enamel surfaces
Mitra Shabanian1, Seyed Ebrahim Jabarifar, Shirin Salavati
1Department of Restorative Dentistry, University of Western Australia, Nedlands, WA, Australia.
Purpose:
To evaluate the changes in the enamel surface microhardness following the application of various suspensions of Crest and Pooneh toothpastes with and without fluoride.
Materials And Methods:
Fifty-six enamel blocks of primary incisors were exposed to a pH-cycling regime consisting of demineralisation and remineralisation solution, then suspensions of the dentifrices Crest 1100, Crest 500, Pooneh 500, Pooneh without fluoride. Changes of the enamel surface microhardness in pre-demineralisation (initial), post-demineralisation (demineralised) and post-remineralisation (final) stages were measured for four groups and were analysed using the Student t test and one-way ANOVA.
Results:
The percentages of changes in surface microhardness for Crest 1100, Crest 500, Pooneh 500 and Pooneh without fluoride were 45.4, 35.4, 28.6 and 23.7, respectively.
Conclusion:
Average changes of surface microhardness for Crest 1100 were significantly higher than Crest 500, Pooneh 500 and Pooneh without fluoride.
Related Concept Videos
Minerals
Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin and...
Development of the Oral Microbiota
Tooth Anatomy
The Crown, Neck, and Root
The visible part of the tooth is referred to as the crown. It's covered by enamel, the hardest substance in the human body. The crown is uniquely shaped for each type of tooth, allowing for different functions such as cutting, tearing, or grinding food.
