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Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...

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Related Experiment Video

Updated: May 19, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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Hsp110 is required for spindle length control.

Taras Makhnevych1, Philip Wong, Oxana Pogoutse

  • 1Department of Biochemistry, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 1A8, Canada.

The Journal of Cell Biology
|August 22, 2012
PubMed
Summary
This summary is machine-generated.

Heat shock proteins (Hsp70/Hsp110) play a crucial role in cell division by regulating kinesin motors. This research reveals their essential function in ensuring proper spindle assembly during the S phase of the cell cycle.

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Last Updated: May 19, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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10:52

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Published on: August 13, 2016

Area of Science:

  • Cell biology
  • Molecular biology
  • Protein interactions

Background:

  • Heat shock proteins (Hsp70/Hsp110) are critical molecular chaperones involved in various cellular processes.
  • Understanding their specific roles in complex cellular machinery like spindle assembly is essential.

Purpose of the Study:

  • To identify novel functions of Hsp70/Hsp110 chaperones within the cell.
  • To elucidate the specific role of Hsp110 in spindle assembly and its interaction with kinesin motors.

Main Methods:

  • Systematic affinity purification coupled with mass spectrometry to map chaperone-protein interactions.
  • Analysis of the Hsp70-Hsp110 chaperone complex and its role in kinesin-5 motor activity.

Main Results:

  • A comprehensive chaperone-protein network of 1,227 interactions involving 9 chaperones and 473 proteins was established.
  • Hsp110 was found to be essential for spindle assembly by modulating the kinesin-5 motor Cin8.
  • Hsp110 Sse1, acting as a nucleotide exchange factor for Hsp70 chaperones (Ssa1/Ssa2), is required for correct kinesin-5 motor distribution and bipolar spindle formation in S phase.

Conclusions:

  • The Hsp70-Hsp110 chaperone complex antagonizes Cin8 motor activity, preventing premature spindle elongation during S phase.
  • This study reveals a novel mechanism for Hsp70/Hsp110 involvement in cell division and spindle dynamics.