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Updated: May 19, 2026

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Resolution of a complex ionic mixture of an apparently homogenous protein preparation by preparative electrophoresis
1Biomolecular Research Institute, 343 Royal Parade, Parkville, Victoria 3052, Australia. ashokdubey@nsit.ac.in
Abstract:
Preparations of recombinant envelope glycoprotein E2 of hepatitis C virus (r-HCV E2), found to be homogeneous by N-terminal amino acid sequencing and mass spectrometry, resolved into multiple ionic species (isoforms) when analysed by isoelectric focusing (IEF) gel electrophoresis in the p1 range of 3-10. These isoforms possessed pI values in the range of 4.5-8.2. The major isoform with p1 value of approximately 7.1 was separated from the rest of them by employing a method developed on Gradiflow BF 200, a device based on preparative electrophoresis. This isoform was adjudged to be homogenous by IEF and by native polyacrylamide gel electrophoresis (PAGE).
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