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Heparin-like surface modification of polyethersulfone membrane and its biocompatibility
Min Tang1, Jimin Xue, Kelin Yan
1College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
Journal of Colloid and Interface Science
|August 23, 2012
Summary
This study modified polyethersulfone (PES) membranes with sulfonic acid and carboxylic acid groups, creating a heparin-like surface. The modified membranes demonstrated reduced protein adsorption, suppressed platelet adhesion, and enhanced cytocompatibility, indicating improved biocompatibility.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Surface Modification
Background:
- Polyethersulfone (PES) membranes are widely used but can cause adverse biological responses.
- Improving the biocompatibility of PES membranes is crucial for biomedical applications.
- Heparin-like surfaces are known to reduce thrombogenicity and protein adsorption.
Purpose of the Study:
- To modify PES membranes with sulfonic acid and carboxylic acid groups to create a heparin-like surface.
- To evaluate the impact of this modification on protein adsorption, platelet adhesion, and blood compatibility.
- To assess the cytocompatibility of the modified membranes using hepatocytes.
Main Methods:
- Sulfonated polyethersulfone (SPES) and poly(acrylonitrile-co-acrylic acid-co-vinyl pyrrolidone) (P(AN-AA-VP)) were synthesized.
- PES membranes were modified by blending with SPES and P(AN-AA-VP) via phase-inversion.
- Protein adsorption (BSA, FBG), platelet adhesion, blood coagulation (TAT, APTT), complement activation (C3a, C5a), and hepatocyte function (SEM, MTT) were analyzed.
Main Results:
- Modified membranes exhibited significantly reduced adsorption of bovine serum albumin (BSA) and bovine serum fibrinogen (FBG).
- Platelet adhesion, thrombin-antithrombin (TAT) generation, CD62p expression, and complement activation (C3a, C5a) were suppressed on modified membranes.
- Modified membranes prolonged activated partial thromboplastin time (APTT) and showed improved hepatocyte function and viability.
- Scanning electron microscope (SEM) and MTT assays confirmed enhanced cytocompatibility of modified membranes.
Conclusions:
- Surface modification of PES membranes with sulfonic and carboxylic acid groups creates a heparin-like surface with excellent biocompatibility.
- The modified membranes demonstrate reduced thrombogenicity and protein adsorption, making them promising for biomedical applications.
- Heparin-like surface modification is an effective strategy to enhance the biocompatibility of polymeric membranes.
