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Updated: May 19, 2026

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Nanoscale programmable sequence-specific patterning of DNA scaffolds using RecA protein.

R Sharma¹, A G Davies, C Wälti

¹School of Electronic and Electrical Engineering, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.

|August 23, 2012

Summary

Researchers used the protein RecA for precise nanoscale patterning of double-stranded DNA (dsDNA). This method achieves over 85% yield, enabling applications in molecular and nanoscale assembly.

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Area of Science:

Biotechnology
Molecular Biology
Nanotechnology

Background:

Molecular self-assembly is key for integrating nanoscale components into larger devices.
Protein-DNA interactions offer precise control over molecular positioning.

Purpose of the Study:

To utilize the protein RecA for programmable patterning of double-stranded DNA (dsDNA).
To achieve molecular-scale precision in positioning nanoscale objects on DNA scaffolds.

Main Methods:

Employing the protein RecA to form nucleoprotein filaments on dsDNA scaffolds.
Utilizing sequence homology between single-stranded DNA (ssDNA) and target dsDNA regions for binding RecA.

Main Results:

Achieved a patterning yield exceeding 85% for dsDNA molecules.
Demonstrated concurrent patterning at multiple locations on a single dsDNA scaffold.
Confirmed separation between assembled nucleoprotein filaments of less than 4 nm.

Conclusions:

RecA protein enables highly efficient and programmable DNA scaffold patterning.
The technique offers molecular-scale precision for nanoscale assembly.
This method is a crucial step towards advanced molecular and nanoscale assembly applications.