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Related Concept Videos

Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

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Related Experiment Video

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Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies
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Published on: June 30, 2023

Constructing the angiome: a global angiogenesis protein interaction network.

Liang-Hui Chu1, Corban G Rivera, Aleksander S Popel

  • 1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205, USA. lchu5@jhu.edu

Physiological Genomics
|August 23, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed GeneHits, a novel system to map protein interactions in angiogenesis. This angiome network aids in understanding blood vessel formation and identifying therapeutic targets for diseases like cancer.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Bioinformatics

Background:

  • Angiogenesis, the formation of new blood vessels, is crucial for development and implicated in diseases like cancer and AMD.
  • Dysregulation of angiogenesis contributes to various pathologies, necessitating a deeper understanding of its regulatory mechanisms.
  • Current knowledge gaps hinder the development of effective therapeutic strategies for angiogenesis-related disorders.

Purpose of the Study:

  • To identify key proteins involved in angiogenesis.
  • To construct a comprehensive protein-protein interaction network (angiome) for angiogenesis.
  • To identify signaling networks driving and inhibiting angiogenesis for therapeutic targeting.

Main Methods:

  • Developed GeneHits, an integrative framework for high-throughput data analysis.
  • Constructed an initial angiome network (478 proteins, 1,488 interactions) and an extended version (1,233 proteins, 5,726 interactions).
  • Validated the network using cross-validation and gene expression datasets; analyzed topological properties and functional enrichment.

Main Results:

  • The angiome network provides a detailed map of protein interactions in angiogenesis.
  • Analysis identified growth factor signaling networks driving angiogenesis and antiangiogenic signaling networks.
  • The extended angiome offers a more complete interaction map.

Conclusions:

  • The GeneHits system and constructed angiome networks advance the understanding of angiogenesis.
  • Identified signaling pathways offer potential therapeutic targets for angiogenesis-related diseases.
  • The findings provide candidates for experimental validation in disease treatment.