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[Comparative analysis of 2 methods of DNA extraction]
I Sánchez García1, C Martín Seisdedos, R Pérez Grande
1Departamento de Medicina, Hospital Clínico Universitario, Salamanca.
Sangre
|October 1, 1990
Abstract:
The study of the organization of immunoglobulin and T cell receptor genes in leukaemias and lymphomas depends on our ability to obtain high molecular weight DNA. We have compared two different methods of DNA extraction: one being enzymatic, using the enzyme proteinase K, and the other chemical, using urea as a substance purifying DNA. Since the amount and purity of the DNA obtained are similar with each of the two methods of DNA extraction, both are valid to make such a genetic analysis.