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Updated: May 19, 2026
10:08
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
Published on: May 21, 2021
Self-packed filter plates: a good alternative for pre-packed filter plates for developing purification processes for
Xiaonan Li1, Guy de Roo, Kim Burgers
1Synthon BV, Downstream Processing, Nijmegen, The Netherlands.
Biotechnology Journal
|August 23, 2012
Summary
Self-packed filter plates are validated for high-throughput screening (HTS) in therapeutic protein purification. The Seahorse plate demonstrated superior performance, enabling accurate resin dispensing for efficient downstream process development.
Area of Science:
- Biotechnology
- Chemical Engineering
- Biopharmaceutical Manufacturing
Background:
- High-throughput screening (HTS) accelerates downstream process development for therapeutic proteins.
- Existing methods require optimization for efficient resin handling in HTS applications.
Purpose of the Study:
- To develop and validate a controlled method for dispensing resin into self-packed filter plates for HTS.
- To identify the most suitable filter plate for HTS-based purification process development.
Main Methods:
- Tested 13 different filter plates for HTS suitability.
- Established optimal conditions for preparing self-packed plates with POROS 50HS resin.
- Validated dispensing accuracy using UV adsorption at 420 nm.
Main Results:
- The Seahorse plate was identified as the optimal filter plate for HTS applications.
- Linear correlations were achieved between resin concentration and UV adsorption.
- Dispensing accuracy was controlled within a 1% deviation.
Conclusions:
- Self-packed filter plates are suitable for HTS-based purification process development.
- The validated method ensures accurate and reproducible resin dispensing.
- This approach enhances the efficiency of biopharmaceutical downstream processing.
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