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Related Experiment Video

Updated: May 19, 2026

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
16:38

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

Published on: November 26, 2012

GABA release by hippocampal astrocytes.

Karim Le Meur1, Juan Mendizabal-Zubiaga, Pedro Grandes

  • 1INSERM U603 Paris, France.

Frontiers in Computational Neuroscience
|August 23, 2012
PubMed
Summary

Hippocampal astrocytes release the inhibitory neurotransmitter gamma-amino-butyric acid (GABA), influencing neuronal activity. This study characterizes this astrocyte-to-neuron signaling and its dual excitatory-inhibitory impact.

Keywords:
GABAgliagliotransmissionglutamategranule cellspyramidal cellstaurine

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Last Updated: May 19, 2026

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Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Published on: March 11, 2020

Area of Science:

  • Neuroscience
  • Cell Biology
  • Neurochemistry

Background:

  • Astrocytes modulate neuronal function via transmitter release.
  • Astrocyte-derived gamma-amino-butyric acid (GABA) release is poorly understood.
  • Glutamate and ATP are known astrocyte-derived transmitters.

Purpose of the Study:

  • To investigate whether hippocampal astrocytes release GABA.
  • To characterize the properties of astrocyte-derived GABAergic signaling.
  • To determine the extent of GABA expression in hippocampal astrocytes.

Main Methods:

  • Whole-cell recordings in rat acute brain slices.
  • Electron microscopy of immunostained hippocampal sections.
  • Analysis of spontaneous inhibitory currents in principal neurons.

Main Results:

  • Spontaneous, slow inhibitory currents activating GABA(A) receptors were observed in hippocampal neurons.
  • These currents exhibited non-synaptic characteristics, similar to astrocytic glutamate release.
  • Osmotic pressure reduction increased non-synaptic GABA and glutamate currents.
  • Approximately 80% of hippocampal astrocytes expressed GABA.

Conclusions:

  • Hippocampal astrocytes release GABA, mediating inhibitory neurotransmission to principal neurons.
  • This astrocyte-derived GABAergic signaling contributes to the dual excitatory and inhibitory influence of astrocytes on the hippocampal network.
  • The functional significance of astrocytic GABA and glutamate release in hippocampal pathophysiology requires further investigation.