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08:39
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
Published on: December 5, 2011
Large-scale animal cell cultures: design and operational considerations
K Ramasubramanyan1, K Venkatasubramanian
1Dept. of Chemical and Biochemical Engineering, Rutgers, State University of New Jersey, Piscataway 08855.
Advances in Biochemical Engineering/Biotechnology
|January 1, 1990
Summary
Scaling up animal cell culture for biological manufacturing requires preserving protein activity. This study emphasizes a rational, integrated systems approach considering both biological and engineering factors for successful bioreactor design and operation.
Area of Science:
- Biotechnology
- Bioprocess Engineering
- Cell Culture Technology
Background:
- Large-scale animal cell culture is increasingly vital for biological manufacturing, particularly for protein production.
- Current methods necessitate a structured approach to bioreactor design and scale-up.
- Maintaining the biological activity of high-value biomolecules during scale-up is paramount.
Purpose of the Study:
- To outline a rational strategy for the design and scale-up of bioreactors for animal cell culture.
- To emphasize the preservation of biological activity as the central goal in scaling up bioprocesses.
- To highlight the critical interplay between biological and engineering considerations.
Main Methods:
- Discussion of bioreactor design principles tailored for animal cell processes.
- Analysis of operational parameters crucial for maintaining cell viability and product integrity.
- Integration of biological and engineering perspectives for a holistic approach.
Main Results:
- A framework for rational bioreactor design and scale-up is presented.
- The importance of preserving the biological activity of manufactured proteins is underscored.
- Key biological and engineering factors influencing successful scale-up are identified.
Conclusions:
- An integrated systems approach is essential for the successful scale-up of animal cell culture processes.
- Balancing biological requirements with engineering constraints ensures optimal bioprocess performance.
- This strategy supports the efficient and effective manufacture of biologicals.
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