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Skeletal keratan sulfate chain molecular weight calibration by high-performance gel-permeation chromatography
J M Dickenson1, H G Morris, I A Nieduszynski
1Division of Biological Sciences, Lancaster University, Bailrigg, United Kingdom.
Analytical Biochemistry
|November 1, 1990
Abstract:
A method has been developed for the molecular sizing of skeletal keratan sulfate chains using an HPLC gel-permeation chromatography system. Keratan sulfate chains and keratanase-derived oligosaccharides were prepared from the nucleus pulposus of bovine intervertebral disc (6-year-old animals). A Bio-Gel TSK 30 XL column eluted in 0.2 M NaCl and at 30 degrees C was calibrated with keratan sulfate oligosaccharides of known size as well as 3H-end-labeled keratan sulfate chains to yield the relationship.