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Updated: May 19, 2026
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
Published on: February 23, 2020
A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA)
Junhai Kai1, Aniruddha Puntambekar, Nelson Santiago
1Siloam Biosciences Inc., 413 Northland Blvd, Cincinnati, USA. Jkai@Siloambio.com
Abstract:
In this work we introduce a novel microfluidic enzyme linked immunoassays (ELISA) microplate as the next generation assay platform for unparalleled assay performances. A combination of microfluidic technology with standard SBS-configured 96-well microplate architecture, in the form of microfluidic microplate technology, allows for the improvement of ELISA workflows, conservation of samples and reagents, improved reaction kinetics, and the ability to improve the sensitivity of the assay by multiple analyte loading. This paper presents the design and characterization of the microfluidic microplate, and its application in ELISA.
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