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Updated: May 19, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
Published on: November 1, 2012
Structure-based design of a highly constrained nucleic acid analogue: improved duplex stabilization by restricting
Stephen Hanessian1, Benjamin R Schroeder, Robert D Giacometti
1Department of Chemistry, Université de Montréal, QC, Canada. stephen.hanessian@umontreal.ca
Abstract:
Dual conformational restriction: a new, highly constrained modification of the α-L-locked nucleic acid (α-L-LNA) scaffold that locks the sugar furanose ring in an N-type configuration and also restricts rotation around torsion angle γ was synthesized. This new modification increases the thermostability of an oligonucleotide duplex compared to using a single mode of constraint alone.
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