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Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.

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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
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Flavivirus replication complex assembly revealed by DNAJC14 functional mapping.

Zhigang Yi1, Zhenghong Yuan, Charles M Rice

  • 1Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, USA.

Journal of Virology
|August 24, 2012
PubMed
Summary
This summary is machine-generated.

DNAJC14 protein regulates flavivirus replication by targeting viral proteins to specific membranes. This chaperone action forms a protein scaffold essential for replication complex assembly and function.

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Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • DNAJC14, an Hsp40 family protein, influences flavivirus replication.
  • The precise role of DNAJC14 in flavivirus replication complex (RC) formation remains unclear.
  • Both insufficient and excessive DNAJC14 levels inhibit viral replication.

Purpose of the Study:

  • To elucidate the mechanism of DNAJC14's involvement in flavivirus RC formation.
  • To investigate the domains of DNAJC14 critical for its function.
  • To propose a model for DNAJC14's role in viral replication.

Main Methods:

  • Utilized yellow fever virus (YFV) as a model system.
  • Investigated protein redistribution and clustering using microscopy.
  • Employed mutagenesis to identify functional domains.
  • Analyzed protein interactions within detergent-resistant membranes.

Main Results:

  • DNAJC14 targets YFV nonstructural proteins to detergent-resistant membranes via its transmembrane and membrane-binding domains (MBD).
  • DNAJC14 and the YFV RC form a stable protein interaction network resistant to Triton solubilization.
  • The C-terminal self-interaction domain is crucial for DNAJC14's incorporation into this network.
  • Fusion of DNAJC14's MBD and self-interaction domain to another Hsp40 protein conferred YFV inhibitory activity.

Conclusions:

  • DNAJC14 acts via specific membrane targeting and protein-protein interactions to facilitate YFV RC formation.
  • A novel model suggests DNAJC14 mediates a microdomain-specific chaperone event.
  • This process remodels the RC membrane and establishes a protein scaffold for viral replication.