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Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

Microorganisms in Medicine and Therapeutics

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

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Updated: May 19, 2026

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Progress on RNAi-based molecular medicines.

Jing Chen¹, Jianping Xie

¹Institute of Modern, Biopharmaceuticals, State Key, Laboratory Breeding Base of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.

International Journal of Nanomedicine

|August 24, 2012

Summary

This summary is machine-generated.

RNA interference (RNAi) offers a powerful method for gene silencing in medicine. This review covers RNAi drug development, delivery systems, and clinical progress for treating diseases.

Keywords:

RNA interference delivery systems liposome molecular medicines nanoparticle

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Area of Science:

Molecular Medicine
Gene Therapy
Drug Development

Background:

RNA interference (RNAi) is a natural process for post-transcriptional gene silencing.
RNAi technology offers therapeutic potential by suppressing disease-relevant gene expression.
The simplicity and stability of RNAi present significant advantages in molecular medicine.

Purpose of the Study:

To review current research on RNA interference (RNAi) in molecular medicine.
To summarize advancements in delivery systems for RNAi-based therapeutics.
To provide a compendium of RNAi drugs currently in clinical development.

Main Methods:

Literature review of ongoing research endeavors in RNAi.
Analysis of various delivery systems including nanoparticles, viral vectors, and bacteriophages.
Compilation of data on RNAi drugs in different clinical trial phases.

Main Results:

Several RNAi-based drugs are progressing through clinical trials.
Diverse delivery systems are being explored to enhance RNAi efficacy and safety.
Ongoing research focuses on target discovery and validation for RNAi therapeutics.

Conclusions:

RNA interference (RNAi) is a rapidly advancing field with significant therapeutic promise.
Effective delivery systems are crucial for the clinical success of RNAi-based drugs.
Continued research and development are expected to yield new RNAi treatments for various diseases.