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Related Experiment Videos

[Structure, expression and control of calbindins-D].

M Thomasset1, N Lomri, F L'horset

  • 1Inserm U120, Alliée CNRS, Le Vesinet.

Annales D'Endocrinologie
|January 1, 1990
PubMed
Summary
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The CaBP9K gene shows tissue-specific expression in rats, regulated by vitamin D in the intestine and estrogen in the uterus. This calcium-binding protein gene is a model for steroid hormone gene regulation.

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Genetics

Background:

  • Calbindins-D (CaBP9K and 28K) are high-affinity intracellular calcium-binding proteins.
  • CaBP9K and CaBP28K are encoded by distinct genes without direct genetic linkage.
  • The tissue-specific expression and regulation of the CaBP9K gene are investigated.

Purpose of the Study:

  • To elucidate the tissue-specific expression and hormonal regulation of the rat CaBP9K gene.
  • To identify regulatory elements within the CaBP9K gene promoter.
  • To establish CaBP9K as a model for studying steroid hormone-mediated gene expression.

Main Methods:

  • Analysis of CaBP9K gene expression in rat tissues (intestine, placenta, uterus).
  • Investigation of the effects of 1,25(OH)2D3 and calcium on CaBP9K mRNA in vitamin D-deficient rats.

Related Experiment Videos

  • Assessment of estrogen's impact on CaBP9K gene transcription in the uterus.
  • Characterization of the CaBP9K gene promoter region for regulatory sequences.

    • Main Results:

    • CaBP9K gene is expressed in rat intestine, placenta, and uterus as a 0.5kb transcript.
    • 1,25(OH)2D3 rapidly induces CaBP9K mRNA synthesis and accumulation in the duodenum.
    • Calcium also stimulates CaBP9K gene expression in the intestine.
    • Estrogen, not 1,25(OH)2D3, stimulates CaBP9K gene transcription in the uterus.
    • The CaBP9K promoter contains TATA, CAAT sequences, and steroid hormone response elements.

    Conclusions:

    • The CaBP9K gene exhibits distinct tissue-specific expression patterns.
    • Its expression is differentially regulated by vitamin D and calcium in the intestine, and by estrogen in the uterus.
    • The rat CaBP9K gene serves as a valuable model for dissecting tissue-specific gene regulation by steroid hormones.