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Quantifying monocyte infiltration in response to intradermal tetanus toxoid injection
Joanna Z Peng1, David E Gutstein, Lisa Beck
1Merck Sharp & Dohme Corp., Whitehouse Station, NJ, USA.
Biomarkers in Medicine
|August 25, 2012
Summary
Recent tetanus toxoid (TT) vaccination enhances the reproducibility of monocyte responses in delayed-type hypersensitivity reactions. This finding aids in developing new therapies for inflammatory disorders.
Area of Science:
- Immunology
- Dermatology
Background:
- Delayed-type hypersensitivity (DTH) reactions are crucial in cell-mediated immunity.
- Monocyte recruitment to the skin is a key component of DTH responses.
Purpose of the Study:
- To characterize monocyte behavior during a DTH reaction induced by intradermal tetanus toxoid (TT).
- To assess the impact of recent TT vaccination on monocyte response reproducibility.
Main Methods:
- Men with positive anti-tetanus titers were divided based on recent TT vaccination status.
- Intradermal injections of TT and saline were administered, with skin biopsies collected.
- The procedure was repeated on the contralateral side after two weeks.
Main Results:
- Individuals vaccinated within one month showed more consistent and less variable monocyte responses.
- Monocyte concentration peaked at 48 hours post-injection in recently revaccinated subjects.
Conclusions:
- A novel assay quantifies dermal monocyte/macrophage influx.
- This method can evaluate therapies for inflammatory conditions involving monocyte recruitment, such as DTH.
