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Updated: May 19, 2026
Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
Published on: April 12, 2019
Alkaline phosphatase activity in human colostrum as a valuable predictive biomarker for lactational mastitis in
Ljiljana Bjelakovic1, Gordana Kocic, Bojko Bjelakovic
1Department of Hygiene, Faculty of Sport and Physical Education, University of Nis, Čarnojevića street 10, Nis, Serbia. ljilja975@gmail.com
Aims:
Biochemical investigations have shown that an indigenous milk enzyme - alkaline phosphatase (ALP) - which is detectable in the lactocytes, plays a very important diagnostic role in clinical medicine, since its activity varies in different tissues and serves as a specific indicator of disease states. The purpose of this study was to evaluate ALP activity in human colostrum as a possible early predictive biomarker for lactational mastitis in nursing mothers.
Patients & Methods:
During a period from May to July 2010, a total of 60 healthy nursing mothers were recruited for this study.
Results:
The mean level of colostrum ALP activity from the affected breasts was significantly higher when compared with ALP activity from the contralateral asymptomatic as well as 'healthy' breasts (p < 0.01).
Conclusion:
Determining ALP activity in colostrum could be a valuable biochemical marker for an early prediction of mastitis in nursing mothers.
