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Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
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Corrigendum to "Humanised mice have functional human neutrophils" [Journal of Immunological Methods, Volume 385, Issues 1-2, 30 November 2012, Pages 96-104].

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Detecting Migration and Infiltration of Neutrophils in Mice
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Published on: February 6, 2020

Humanised mice have functional human neutrophils.

Alice M Coughlan1, Simon J Freeley, Michael G Robson

  • 1King's College London, King's Health Partners, Medical Research Council Centre for Transplantation, Guy's Hospital, London SE1 9RT, UK.

Journal of Immunological Methods
|August 25, 2012
PubMed
Summary

Researchers developed a humanized mouse model using human stem cells to study human neutrophils in vivo. This model enables functional analysis of human neutrophils, advancing research in infection and autoimmunity.

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Published on: July 10, 2013

Area of Science:

  • Immunology
  • Hematology
  • Transplantation Biology

Background:

  • Murine neutrophil models have limitations for human disease research.
  • A need exists for in vivo models utilizing human neutrophils.

Purpose of the Study:

  • To establish a humanized mouse model for studying human neutrophil function in vivo.
  • To assess the functional capacity of human neutrophils derived from cord blood stem cells in a murine host.

Main Methods:

  • Human CD34+ stem cells from cord blood were injected into irradiated NOD-scid-γc(-/-) mice.
  • Granulocyte-colony stimulating factor (GCSF) was used to mobilize human neutrophils.
  • Lipopolysaccharide (LPS) was administered to induce inflammatory responses.
  • Flow cytometry was used to analyze neutrophil surface markers (L-selectin, CD66b, CD11b, CD63).
  • Functional assays included respiratory burst and degranulation in response to fMLP and Escherichia coli.

Main Results:

  • Humanized mice successfully engrafted with human CD34+ stem cells.
  • GCSF treatment mobilized circulating human neutrophils exhibiting activation markers.
  • LPS challenge induced further neutrophil activation, L-selectin downregulation, and lung sequestration.
  • Human neutrophils demonstrated robust functional responses, including respiratory burst and degranulation.

Conclusions:

  • Functional human neutrophils can develop from CD34+ cord blood stem cells in NOD-scid-γc(-/-) mice.
  • This humanized mouse model is a valuable tool for in vivo studies of human neutrophils.
  • The model holds promise for investigating human neutrophil roles in infection and autoimmunity.