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Blood cells attachment after root conditioning and PRP application: an in vitro study
Andrea Abi Rached Dantas1, Lucas Amaral Fontanari, Eduardo de Paula Ishi
1Department of Operative Dentistry, School of Dentistry at Araraquara, State University of Sao Paulo, UNESP, 1680 Humaita Street, Araraquara, 14801-903, Sao Paulo, Brazil. aarached@yahoo.com.br
The Journal of Contemporary Dental Practice
|August 25, 2012
Summary
Citric acid effectively removes smear layer and exposes collagen for periodontal regeneration. Platelet-rich plasma (PRP) application with citric acid enhanced blood cell adhesion, optimizing healing.
Area of Science:
- Periodontal regeneration
- Biomaterials science
- Cell adhesion
Background:
- Root conditioning aims to remove the smear layer and expose collagen.
- This process is crucial for promoting periodontal regeneration.
- Platelet-rich plasma (PRP) may enhance healing by influencing blood cell adhesion.
Purpose of the Study:
- To assess smear layer removal and collagen exposure after root conditioning.
- To evaluate the effect of PRP on blood cell adhesion to the root surface.
- To compare the efficacy of different conditioning agents.
Main Methods:
- 160 scaled root samples were divided into five groups and treated with saline, EDTA, citric acid, tetracycline hydrochloride, or sodium citrate.
- Root surface modification and blood elements adhesion were evaluated using scanning electron microscopy (SEM).
- PRP gel was applied to assess fibrin clot formation and blood cell adhesion.
Main Results:
- Citric acid (25%) demonstrated superior performance in smear layer removal and collagen exposure (RSMI).
- Citric acid also showed the highest blood elements adhesion index (BEAI).
- Fibrin clot formation was exclusively observed in the citric acid group after PRP application.
Conclusions:
- Citric acid is the most effective root conditioner for smear layer removal and collagen exposure.
- Citric acid combined with PRP application promotes blood cell adhesion, potentially optimizing periodontal healing.
- Root conditioning followed by PRP application shows promise for enhancing periodontal regeneration.
