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Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...

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Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
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Structural biology of replication initiation factor Mcm10.

Wenyue Du1, Melissa E Stauffer, Brandt F Eichman

  • 1Departments of Biological Sciences and Biochemistry, Center for Structural Biology, Vanderbilt University, Nashville, TN, 37232, USA, wenyue.du@vanderbilt.edu.

Sub-Cellular Biochemistry
|August 25, 2012
PubMed
Summary

Minichromosome maintenance protein 10 (Mcm10) is crucial for eukaryotic DNA replication fork assembly. Its modular structure suggests Mcm10 acts as a scaffold, aiding in DNA unwinding and coordinating replication enzymes.

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Minichromosome maintenance protein 10 (Mcm10) is a key non-enzymatic factor in eukaryotic DNA replication.
  • Mcm10 interacts with DNA and other replication proteins, facilitating pre-replicative complex activation and origin recruitment.
  • Its precise role in coordinating helicase and polymerase activities during replication fork progression is under investigation.

Purpose of the Study:

  • To elucidate the functional mechanisms of Mcm10 in eukaryotic DNA replication.
  • To understand how Mcm10's modular architecture contributes to its role as a replisome scaffold.
  • To define Mcm10's potential involvement in origin DNA melting, DNA polymerase alpha recruitment, and enzymatic activity coordination.

Main Methods:

  • Biochemical characterization of Mcm10.
  • Structural analysis of Mcm10 from various organisms.
  • Investigating Mcm10 interactions with DNA and replication proteins.

Main Results:

  • Mcm10 possesses a modular architecture enabling its function as a replisome scaffold.
  • Evidence suggests Mcm10 interacts with both single- and double-stranded DNA, as well as DNA polymerase alpha and Mcm2-7 complex.
  • Mcm10 is vital for activating the pre-replicative complex and recruiting proteins to replication origins.

Conclusions:

  • Mcm10's modular structure is key to its function as a scaffold in the replisome.
  • Mcm10 likely plays a direct or indirect role in DNA unwinding and coordinating replication machinery during S-phase.
  • Further research into Mcm10's interactions will clarify its precise mechanisms in DNA replication elongation.