Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proofreading01:31

Proofreading

Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase Enzyme
Proofreading01:43

Proofreading

Overview
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A thumb-domain insertion balances processivity and fidelity in DNA polymerase ε.

Nucleic acids research·2026
Same author

Evidence for a functional interaction between yeast Pol ε and PCNA in vivo.

Nucleic acids research·2025
Same author

Decoding nucleoside supplementation: how thymidine outperforms ribonucleosides in accelerating mammalian replication forks.

Nucleic acids research·2025
Same author

Management of pregnancy and childbirth in Glanzmann thrombasthenia: A case series and review.

British journal of haematology·2025
Same author

Impact of a Maternal Medicine Hub on post-partum haemorrhage in women with inherited bleeding disorders: A retrospective service evaluation.

Obstetric medicine·2025
Same author

The POLγ Y951N patient mutation disrupts the switch between DNA synthesis and proofreading, triggering mitochondrial DNA instability.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Future Directions in Biotechnological and Pharmacological Applications of CAIs.

Sub-cellular biochemistry·2026
Same journal

Industrial and Environmental Applications of Carbonic Anhydrases.

Sub-cellular biochemistry·2026
Same journal

Applications of Carbonic Anhydrase Inhibitors in Arthritis, Neuropathic Pain, Acute Mountain Sickness, and Cerebral Ischemia.

Sub-cellular biochemistry·2026
Same journal

Applications of Carbonic Anhydrase Inhibitors in Neurological Disorders, Mechanisms and Therapeutic Potential.

Sub-cellular biochemistry·2026
Same journal

Carbonic Anhydrase Inhibitors in Oncology.

Sub-cellular biochemistry·2026
Same journal

Therapeutic Applications of Carbonic Anhydrase Inhibitors in Ophthalmology.

Sub-cellular biochemistry·2026
See all related articles

Related Experiment Video

Updated: May 19, 2026

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

DNA polymerase ε.

Matthew Hogg1, Erik Johansson

  • 1Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, SE-90187, Sweden.

Sub-Cellular Biochemistry
|August 25, 2012
PubMed
Summary
This summary is machine-generated.

DNA polymerase ε (Pol ε) is crucial for eukaryotic DNA replication, playing key roles in initiation and leading strand synthesis. This chapter details its structure, subunits, and functions.

More Related Videos

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
12:48

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Related Experiment Videos

Last Updated: May 19, 2026

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
12:48

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA polymerase ε (Pol ε) is one of three replicative DNA polymerases in eukaryotic cells.
  • Pol ε is a multi-subunit enzyme with diverse functions in DNA replication.

Purpose of the Study:

  • To discuss the structure of DNA polymerase ε.
  • To elucidate the function of individual subunits of Pol ε.

Main Methods:

  • Literature review of recent studies on Pol ε.
  • Analysis of structural and functional data of Pol ε subunits.

Main Results:

  • Pol ε is essential for the initiation of DNA replication in yeast.
  • Pol ε participates in leading strand DNA synthesis.

Conclusions:

  • Understanding the structure and subunit functions of Pol ε is key to comprehending DNA replication.
  • Further research into Pol ε will illuminate its critical roles in maintaining genomic stability.