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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: May 19, 2026

Ferric Chloride-induced Murine Thrombosis Models
10:37

Ferric Chloride-induced Murine Thrombosis Models

Published on: September 5, 2016

Small RNAs as potential platelet therapeutics.

Leonard C Edelstein1, Paul F Bray

  • 1The Cardeza Foundation for Hematologic Research and the Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Curtis Building, Room 324, 1015 Walnut Street, Pennsylvania, PA 19107, USA.

Handbook of Experimental Pharmacology
|August 25, 2012
PubMed
Summary

MicroRNAs (miRNAs) regulate gene expression and are crucial for blood cell development. Platelet miRNAs show therapeutic potential as biomarkers and drug delivery agents, offering new avenues for disease treatment.

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Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
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Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

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Last Updated: May 19, 2026

Ferric Chloride-induced Murine Thrombosis Models
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Ferric Chloride-induced Murine Thrombosis Models

Published on: September 5, 2016

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
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Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Published on: September 5, 2016

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Hematology

Background:

  • MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression in mammals.
  • Platelets contain functional miRNA machinery and their miRNA levels correlate with reactivity.
  • Platelet miRNAs target mRNAs involved in platelet function.

Purpose of the Study:

  • To explore the potential of platelet microRNAs (miRNAs) as biomarkers and therapeutic agents.
  • To investigate the use of platelets for targeted delivery of miRNA-based therapies.
  • To examine the role of miRNAs in hematopoietic stem cell gene therapy and platelet storage.

Main Methods:

  • Analysis of miRNA roles in hematopoiesis and megakaryocyte development.
  • Correlation of platelet miRNA levels with platelet reactivity.
  • Investigation of miRNA targeting of platelet function-related mRNAs.
  • Exploration of platelet microparticle release at activation sites.
  • Assessment of cell-preferential miRNA expression for gene therapy.
  • Evaluation of miRNA manipulation in stored platelets.

Main Results:

  • Platelets possess functional miRNA processing and release miRNA-containing microparticles.
  • Platelet miRNAs target mRNAs influencing platelet function.
  • Platelet miRNAs show promise as biomarkers and therapeutic delivery vehicles.
  • miRNA engineering in platelets could enable targeted therapy delivery to sites like tumors and atherosclerotic plaques.
  • Exploiting miRNA expression can enhance lineage-specific gene therapy and improve platelet storage.

Conclusions:

  • Platelet-derived miRNAs hold significant potential as biomarkers and therapeutic agents.
  • Engineering platelets for targeted miRNA delivery offers a novel therapeutic strategy.
  • Further research into miRNA manipulation in platelets could advance gene therapy and transfusion medicine.