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Related Experiment Video

Updated: May 19, 2026

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model
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Fifteen Streptococcus suis serotypes identified by multiplex PCR.

Anusak Kerdsin1, Surang Dejsirilert1, Yukihiro Akeda2

  • 1National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.

Journal of Medical Microbiology
|August 25, 2012
PubMed
Summary

A new multiplex PCR assay effectively identifies 15 Streptococcus suis serotypes in human and pig samples. This method aids in Streptococcus suis serotype surveillance for both human and animal health.

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Area of Science:

  • Veterinary Microbiology
  • Molecular Diagnostics
  • Bacteriology

Background:

  • Streptococcus suis is a significant zoonotic pathogen causing meningitis and other infections in humans and pigs.
  • Accurate and rapid serotyping is crucial for epidemiological surveillance and control of Streptococcus suis outbreaks.
  • Existing methods for Streptococcus suis serotyping can be time-consuming and labor-intensive.

Purpose of the Study:

  • To develop and validate a multiplex polymerase chain reaction (PCR) assay for the simultaneous detection of 15 key Streptococcus suis serotypes.
  • To differentiate between human and pig isolates of Streptococcus suis based on serotype identification.

Main Methods:

  • A multiplex PCR assay was designed and optimized into two reaction sets to cover 15 Streptococcus suis serotypes.
  • The assay was tested using both human and pig isolates, including isolates from Thailand.
  • Specificity was assessed by challenging the assay with other streptococcal species to rule out cross-reactions.

Main Results:

  • The multiplex PCR assay successfully identified nine serotypes (1/2, 1, 2, 3, 4, 7, 9, 14, 16) in the first set and six serotypes (5, 8, 10, 19, 23, 25) in the second set.
  • The assay demonstrated high accuracy in detecting specific serotypes in human (2, 5, 14) and pig (1, 2, 1/2, 3, 4, 5, 7, 9, 14, 16, 19) isolates.
  • No cross-reactivity was observed with other streptococcal species, confirming the assay's specificity.

Conclusions:

  • The developed multiplex PCR assay provides a rapid, specific, and reliable method for identifying multiple Streptococcus suis serotypes.
  • This assay is a valuable tool for the epidemiological surveillance of Streptococcus suis in both human and pig populations.
  • The findings support the use of this assay for timely diagnosis and outbreak investigations involving Streptococcus suis.