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Evaluation of interleukin -1B (+3954) gene polymorphism in patients with chronic and aggressive periodontitis: A
Sujata S Masamatti1, Ashish Kumar, Tarun Kumar A Baron
1Department of Periodontics, I.T.S-CDSR, Murad Nagar, Ghaziabad, Uttar Pradesh, India.
Background:
IL-1 cytokines have central roles in the pathogenesis of periodontal disease. Polymorphism in the locus +3954 (C/T) of the human IL-1B gene has been shown to affect the levels of this cytokine.
Aim:
The aim of the present study was to investigate the association between the IL-1 B (+3954) gene polymorphism and the occurrence of different clinical forms of periodontitis.
Materials And Methods:
Genomic DNA was obtained from 90 individuals and amplified using the PCR with specific primers flanking the locus +3954 of IL-1B. PCR products were submitted to restriction endonuclease digestion and analyzed by gel electrophoresis, allowing for the determination of the genotypes and detection of the polymorphism.
Statistical Analysis:
Fisher's exact test was used for comparing the frequency of genotype distributions between groups.
Results:
The chronic periodontitis group displayed a higher percentage of T alleles (38%) when compared to the aggressive periodontitis group (20%) and to the control group (19%).
Conclusion:
Our study data states that polymorphism in the locus +3954 of IL-1B gene could be a risk factor for chronic periodontitis in a sample of Indian population of Karnataka state.
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