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Different modulating effects of adenosine on neonatal and adult polymorphonuclear leukocytes

Pei-Chen Hou1, Hong-Ren Yu, Ho-Chang Kuo

  • 1Department of Nursing, Kaohsiung Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Niao-Sung district, Kaohsiung 833, Taiwan.

Thescientificworldjournal
|August 25, 2012
PubMed

Insights

Adenosine impairs neonatal polymorphonuclear leukocyte (PMN) functions, unlike in adults. Neonatal PMNs show reduced adhesion, chemotaxis, and reactive oxygen species (ROS) release, suggesting adenosine is not ideal for treating neonatal sepsis.

Area of Science:

  • Immunology
  • Neonatal Biology
  • Cellular Biology

Background:

  • Polymorphonuclear leukocytes (PMNs) are crucial for host defense, with functions including adhesion, migration, phagocytosis, and reactive oxygen species (ROS) release.
  • Adenosine modulates adult PMN functions, but its effects on neonatal PMNs are largely unknown, despite higher adenosine levels in neonatal plasma.

Purpose of the Study:

  • To investigate the effects of adenosine on neonatal polymorphonuclear leukocyte (PMN) functions.
  • To compare the functional responses of neonatal PMNs to adenosine with those of adult PMNs.

Main Methods:

  • Comparative analysis of neonatal and adult PMN functions, including adhesion, chemotaxis, phagocytosis, and ROS production.
  • Assessment of adenosine's modulatory effects on PMN functions, specifically CD11b expression, phagocytosis, chemotaxis, and ROS production.

Main Results:

  • Neonatal PMNs exhibited impaired adhesion, chemotaxis, and ROS production compared to adult PMNs; phagocytosis was not significantly different.
  • Adenosine suppressed CD11b expression in neonatal PMNs, similar to adult PMNs, but did not significantly affect phagocytosis.
  • Adenosine failed to significantly suppress chemotaxis and ROS production in neonatal PMNs, unlike in adult PMNs.

Conclusions:

  • Neonatal PMNs have intrinsic functional deficits in adhesion, chemotaxis, and ROS production.
  • The impaired response of neonatal PMNs to adenosine, potentially due to compromised cellular reactions, suggests that adenosine therapy may not be effective for neonatal sepsis.

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